B Raf/BRAF Antibody orb215918 from Biorbyt

Product Specs
ItemB Raf/BRAF Antibody
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb215918
Quantity10 µg, 100 µg
ApplicationsFC, IHC, WB
Conjugate/TagUnconjugated
FormatLyophilized
ReactivityHuman, Mouse, Rat
TargetB Raf/BRAF
IsotypeRabbit IgG
HostRabbit
ImmunogenE.coli-derived human B Raf recombinant protein (Position: A38-V230). Human B Raf shares 81% amino acid (aa) sequence identity with mouse B Raf
NCBI Full Gene NameB-Raf proto-oncogene, serine/threonine kinase
NCBI Gene AliasesB-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1
ClonalityPolyclonal
Molecular Weight84437 MW
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 µg/ml
StorageStore at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles

Specifications/Features

Application Notes: WB: The detection limit for B Raf is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Application Dilutions: Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat Flow Cytometry, 1-3 µg/1x106 cells, Human

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